A method for analysis of biological materials based on gel electrophoresis using barcode DNA

摘要

Magnetic fine particles bound with a first ligand that specifically binds to a target analyte; And a method for analyzing gel electrophoresis-based biomaterials using nanoparticles comprising a second ligand that specifically binds to a target analyte and at least one barcode DNA. The present invention relates to a method for detecting and analyzing a trace amount of biomolecules without amplification processes such as PCR using nanoparticles containing a large number of barcode DNAs and gel electrophoresis generally used or for detecting sensitive signals . The detection method according to the present invention is capable of detecting miRNAs that are difficult to detect because they are difficult to detect and can detect even minute amounts of atomol concentration (aM; 10 ^-18 M) or 1 젭 mole level and can identify single-base-inconsistent sequences And quantitatively analyzing a plurality of components from multiple complex samples at one time by using barcode DNAs of various sizes. Therefore, the method can be usefully used for diagnosis of cancer and detection of mutation from clinical samples.