Method for induction cardiac myocyte from somatic cells using direct reprogramming strategy by small molecule treatment without genetic modification

摘要

The present invention relates to a pharmaceutical composition comprising a glycogen synthease kinase (GSK) inhibitor, a calcium channel agonist, a cAMP signaling activator, a TGF-β type I receptor inhibitor, and EKR1 a step of culturing a somatic cell in a culture medium containing at least one substance selected from the group consisting of extracellular regulated kinase and Ras-GTP activating protein (Ras-GTPase activating protein) A method for inducing myocardial cells from somatic cells using the same, and a culture medium for inducing differentiation using the same. The present invention having the above-described structure suggests a method of directly reprogramming from somatic cells to chemically induced cardiac myocytes (cicCM) using only low-molecular substances without gene introduction, Can be very useful for the development of a cell therapy agent.