DNA Method for Methylation Detection of Converted DNA by Bisulfite-Treatment Using Inosine-containing Modified Primers

摘要

The present invention relates to a method for detecting methylation using an inosine-containing primer. When methylation-specific primers containing the inosine residue according to the present invention are used, the specificity to the target sequence is higher than that of the conventional methylation-specific primers, and there is no loss in sensitivity, so that even minute amounts of methylated DNA can be efficiently detected have.