DNA SINGLE STRANDED DNA APTAMERS SPECIFICALLY BINDING TO STAPHYLOCOCCUS AUREUS

摘要

A single-stranded DNA extramammary specifically binding to Staphylococcus aureus and a process for its preparation are disclosed. A method for producing an extramammary specifically binding to Staphylococcus aureus comprises a DNA library having DNA primers having PCR primers at both ends and DNAs having 40 to 60 nucleotides randomly arranged in the center, (S1) mixing Staphylococcus aureus into a binding buffer solution to induce Staphylococcus aureus-DNA complex; (S2) of extracting the Staphylococcus aureus-DNA complex by centrifuging a mixture solution of the DNA library and Staphylococcus aureus: Using FACS (fluorescence activated cell sorter), the Salmonella Separating the DNA from the DNA complex (S3); Amplifying the isolated DNA through a polymerase chain reaction (S4); Mixing the amplified DNA with a non-target microorganism in a binding buffer solution to induce a non-target microorganism-DNA complex (S5); And centrifuging the mixed solution of the concentrated DNA and the non-target microorganism to extract DNA not bound to the non-target microorganism (S6).