METHOD FOR DETERMINING cation concentration of zinc in the serum with simultaneous determination of ratio of the cationic zinc and copper in the same sample

摘要

A method for determining the concentration of zinc cations in a sample of blood serum with a simultaneous determination of the ratio of zinc cation and copper cations in the same sample, comprising using as the basic reagent dithizone solution in carbon tetrachloride, incubating the sample of blood serum and standard solution at room temperature in an alkaline medium, removal the organic phase and colorimetry colloidal solutions serum sample and a standard solution containing zinc dithizonate, at 535 nm, calculation of cation concentration s zinc by the formula: C = COPProby / OPStandarta, where C is the molar concentration of the standard solution of zinc sulfate; OPProby - optical density of the sample of blood serum, measured at 535 nm; OPStandarta - optical density of the standard zinc sulphate solution, measured at 535 nm, characterized in that the colorimetry of colloidal solutions of serum sample and a standard solution containing dithizonate copper repeatedly performed at 435 nm and calculation of the ratio of zinc cation and copper in the same sample of serum carried out according to the formula: CC = M / S ∙ (OD / OD) samples / (OD / OD) standard, where C is the molar concentration of the standard solution of copper sulfate; (OD / OD) Samples - ratio of the optical densities of the blood serum samples, measured at 535 nm and 435 nm; (OD / OD) Standard - the ratio of the optical densities of the standard solutions of zinc sulfate and copper sulfate, measured at 535 nm and 435 nm, respectively.