METHOD FOR DETECTION OF TUBERCULOSIS MYCOBACTERIA GENETIC CLUSTER Beijing B0 / W148

摘要

A method for identifying a cluster of mycobacteria tuberculosis Beijing B0 / W148, characterized in that the polymerase chain reaction to amplify DNA fragments of M. tuberculosis chosen from primers P1, P2 and the RE, the sequences of which are presented SEQ ID NO 1, 2, 3, respectively, in the mode of . amplify 98 ° C - 10 min, 30 cycles of 98 ° C - 30 sec, 64 ° C - 30 sec, 72 ° C - 50 sec, 72 ° C - 10 min, 4 ° C, separation of the products.... amplification is carried out by electrophoresis in 2% agarose gel electrophoresis to obtain a fragment of 1021 bp for strains Beijing B0 / W148 or 320 bp in size - for strains not related schihsya to this family.