A method for quantifying methanofullerenes The reaction mixture by UV spectroscopy

摘要

1. A method for the quantitative determination methanofullerenes different degrees of substitution in the reaction mixture by UV-spectroscopy, comprising removing the UV-spectra, the construction of calibration curves based on the values ​​of the second derivative spectrum, by finding them linear regression equations, characterized in that for the sample preparation of the test compounds different substitution degrees of the organic solvents used, recording the UV spectra produced in a wide range of concentrations, the linear regression equation for determination of monosubstituted methanofullerenes h = 1.23 × 10c + 0.01 × 10 disubstituted h = 1.54 × l0c + 0.20 × l0, trisubstituted h = 2.31 × l0c + 0.30 × l0, tetrasubstituted h = 4.00 × l0c + 0.32 × l0, then the determination of methanofullerenes different degrees of substitution in the reaction mixture is sampled analyzed samples from the reaction mixture, removal of UV-spectra plotting based on the second derivative spectrum values, the substitution of the appropriate value h corresponding to a linear regression equation and calculating metanofulerenov concentration in assay mixture .2. A method of quantitative determination methanofullerenes different degrees of substitution in the reaction mixture by UV-spectroscopy according to claim 1, characterized in that as organic solvents are chlorinated benzenes, chlorinated hydrocarbons, toluene, acetonitrile, recording the UV spectra is performed in the concentration range 10-10mol / l, wherein the determined number of components in a mixture of two or more.