Composition for enhancing transgene expression in eukaryotic cells and method for increased production of the target protein encoded by the transgene

摘要

1. A composition for the intensive production of the target protein in eukaryotic cells, comprising a DNA vector with an insert of the target gene protein and the cellular receptor agonist class of receptors PRR - pattern recognition receptors, selected from the following agonists: TLR2 receptor agonists or TLR4 agonists or agonists TLR5 receptor, or TLR7 agonists, TLR8 or receptor agonists or TLR9 agonists or NOD1-receptor agonists or NOD2-receptor agonists, taken in optimal sootnosheniyah.2. A composition according to claim 1, characterized in that the TLR2 receptor agonist used lipoteichoic kislotu.3. A composition according to claim 1, characterized in that the TLR2 receptor agonist used lipopeptid.4. A composition according to claim 1, characterized in that the TLR4 receptor agonist used bacterial lipopolisaharid.5. A composition according to claim 1, characterized in that the TLR4 receptor agonist used acidic peptidoglycan having a molecular weight 1200-40000 kDa.6. A composition according to claim 1, characterized in that the TLR5 receptor agonist used flagellin.7. A composition according to claim 1, characterized in that as a TLR7 receptor agonist used imikvomod.8. A composition according to claim 1, characterized in that as a TLR8 receptor agonist used imikvomod.9. A composition according to claim 1, characterized in that as a TLR7 receptor agonist formulation used CL097, which is a derivative imidazohinolina.10. A composition according to claim 1, characterized in that as a TLR8 receptor agonist formulation used CL097, which is a derivative imidazohinolina.11. A composition according to claim 1, characterized in that the TLR9 receptor agonist used oligonukleo