Process for the preparation and induction of directed differentiation of multipotent cells CULTURE OF LIGHT

摘要

A method of obtaining and induce directed differentiation culture lung multipotent cells, wherein the isolated population of cells from tissue fibrozirovannyh lungs, were cultured for 60 days, twice passaged obtained culture adherent fibrozirovannyh lung cells morphologically representing round cells when adhesion transforming into spindle-shaped cells that express the surface markers CD44, CD73, CD90, CD106, do not express CD31, CD34, CD45, then in the presence of growth factors floor chennye mononuclear cells differentiated in vitro into adipocytes (60 uM indomethacin, 1 ug / ml insulin), chondrocytes (10 ng / ml recombinant human TGF-β1), osteoblasts (10 mM β-glycerophosphate), fibroblasts (2 ug / ml fibroblast growth factor ).