首页> 外国专利> METHOD FOR OBTAINING AN ANTIMICROBIAL, ANTIVIRUS AND IMMUNITY IMMUNTABLE ACTIVITY SUBSTANCE, IN PARTICULAR, WITH RESPECT TO DENDRITIC CELLS, SUBSTANCE OBTAINED BY THIS METHOD, OSETOSEMICALLY RESISTANT

METHOD FOR OBTAINING AN ANTIMICROBIAL, ANTIVIRUS AND IMMUNITY IMMUNTABLE ACTIVITY SUBSTANCE, IN PARTICULAR, WITH RESPECT TO DENDRITIC CELLS, SUBSTANCE OBTAINED BY THIS METHOD, OSETOSEMICALLY RESISTANT

机译:获得针对树突状细胞的特别是针对树突状细胞的抗微生物,抗病毒和免疫活性物质的方法,该物质具有抗局部渗透性

摘要

1. A method of obtaining a substance having antimicrobial, antiviral and immunostimulating activity, in particular, with respect to dendritic cells, which consists in the fact that the crushed plant material, which is used as potato seedlings, is subjected to water extraction, then the aqueous extract is separated from the precipitate, he added a salt agent, followed by concentration of the obtained salt solution by ultrafiltration on a filter with a transmission pore of 300 kilodaltons (CD), after which the solution was frozen for a day, p they are frozen, filtered, the precipitate obtained is removed, and the crude peptidoglycan is precipitated from the solution with an acidic salt agent, followed by treatment with an alkali solution, then the alkaline solution is dialyzed against distilled water on a filter, and peptidoglycan is isolated from the resulting solution by gel permeation chromatography. 2 . The method according to claim 1, characterized in that, as the salt agent, mainly sodium chloride is used. The method according to claim 1, characterized in that an inorganic ammonium salt is used as the acid salt agent in the precipitation step. The method according to claim 1, characterized in that the alkaline solution is dialyzed against distilled water on a filter with 12 KD transmission pores. A substance with antimicrobial, antiviral and immunostimulating activity, in particular with respect to dendritic cells, obtained according to claim 1 and which is a complex biopolymer containing a polysaccharide, peptide and lipid part. The substance according to claim 5, characterized in that the polysaccharide part of the molecule is 40-60% and has the following structure: where m = 3
机译:1.获得特别是对于树突细胞具有抗微生物,抗病毒和免疫刺激活性的物质的方法,其特征在于,将用作马铃薯幼苗的压碎的植物材料进行水提取,然后将水提取物与沉淀物分离,加入盐试剂,然后在具有300道尔顿(CD)传输孔的过滤器上通过超滤浓缩所得盐溶液,然后将溶液冷冻一天,将其冷冻,过滤,除去获得的沉淀物,并用酸性盐试剂从溶液中沉淀出粗肽肽聚糖,然后用碱性溶液处理,然后在过滤器上用蒸馏水对碱性溶液进行透析,并肽肽聚糖通过凝胶渗透色谱法从得到的溶液中分离出。 2。 2.根据权利要求1所述的方法,其特征在于,作为盐剂,主要使用氯化钠。 2.根据权利要求1的方法,其特征在于在沉淀步骤中使用无机铵盐作为酸盐剂。 2.根据权利要求1所述的方法,其特征在于,所述碱性溶液在具有12KD传输孔的过滤器上相对于蒸馏水渗析。 2.根据权利要求1所述的具有抗微生物,抗病毒和免疫刺激活性的物质,特别是对于树突细胞,其是包含多糖,肽和脂质部分的复合生物聚合物。 6.根据权利要求5所述的物质,其特征在于,所述分子的多糖部分为40-60%,并且具有以下结构:其中m = 3

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