SUITABLE FOR USE IN THE ENGINE A COMPLEX METHYL ETHER OF FATTY ACIDS (BiodIESEL) FROM MEETING DIVERS OF SECRET DIVERS SUSPENDED IN THE NATURE AND SEA DIVERS

摘要

1. A method of manufacturing a fatty acid methyl ester (SMEFA) suitable for use in an engine for use as a biodiesel, which comprises the steps of: (i) collecting naturally occurring tin marine microalgae selected from the group consisting of Microspora sp. and Cladophora sp . or cultivated Chlorella variabilis to obtain algae biomass; (ii) drying the biomass in the sun to a residual moisture level of 5-10%; (iii) pretreating the biomass from step (ii) with a steam jet or osmotic shock to destroy cell walls; (iv ) extracting lipids from the algal biomass from step (iii) using hexane as a solvent, or optionally diesel fuel, when the fuel must be used in mixed form to produce crude oil; (v) removing hexane and processing the resulting crude oils fuller earth or, optionally, treating the extract from step (ii) directly with fuller earth to remove phospholipids, pigments and other impurities; (vi) filtering to remove suspended solids and further treating the extract oil from step (v) to reduce the content of free fatty acids (FFA) if it is necessary to obtain a purified oil; (vii) carrying out an alkaline catalyzed transesterification of the purified oil from step (vi), separating the SMEFA and its further purification to obtain suitable for use lzovaniya engine SMEZHK.2. The method according to claim 1, wherein the lipid is extracted from the mud of marine microalgae containing Microspora sp. (ATCC registration number PTA-12197) by extraction with hexane, wherein said lipid has a composition according to gas chromatography