METHOD AND APPARATUS FOR ANALYSIS interactions of biological molecules on biological microchips based on the amino acid residues tryptophan fluorescence

摘要

1. A method of analyzing interactions between molecules fluoresce in the ultraviolet (UV) spectral region, including proteins, peptides, glycoproteins, proteoglycans, and other compounds, the sequence of which contains one or more amino acid residue tryptophan (Trp), and biological molecules, biological cells immobilized in the microchip (biochip), wherein the analysis of interactions performed by measuring the intensity of UV fluorescence triptofana.2 amino acid residues. A method according to claim 1, wherein the cells are immobilized biochip any of the following biological molecules, in any combination:., Nucleic acid molecules, carbohydrates, lipids, proteins, peptides, glycoproteins and proteoglycans, as well as their kompleksov.3. A method according to any one of claims. 1-2, wherein the substrate is coated with a layer of the biochip metalla.4. A method according to any one of claims. 1-2, wherein the biochip substrate consists of quartz stekla.5. A method according to any one of claims. 1-2, wherein the chamber cover material covering the biochip is steklo.6 quartz. A method according to any one of claims. 1-2, wherein the biological molecules immobilized in gel volume cells arranged on podlozhki.7 surface. An apparatus for analysis of interactions between molecules fluoresce in the ultraviolet (UV) region of the spectrum, and biological molecules, biological cells immobilized in the microchip (biochip) - Image biochip analyzer representing a fluorescence microscope suitable for registration in the area of ​​300-400 nm when excited fluorescence in the area of ​​250-300 nm.8. An apparatus for analysis of interactions between molecules in the fluorescent