首页> 外国专利> METHOD OF CREATION OF LINES OF SPRING SOFT WHEAT WITH ELONGATED PERIOD OF EARING AND COMPLEX RESISTANCE TO FUNGAL DISEASES

METHOD OF CREATION OF LINES OF SPRING SOFT WHEAT WITH ELONGATED PERIOD OF EARING AND COMPLEX RESISTANCE TO FUNGAL DISEASES

机译:长发时期和对真菌病的抵抗综合抵抗力强的春小麦生产线的方法

摘要

FIELD: agriculture.;SUBSTANCE: invention relates to agriculture and biotechnology. A hybrid line of spring soft wheat containing a chromosome fragment with two genes from Aegilops speltoides: a gene that determines prolongation of earing (VRN-Asp1), and a gene of resistance to brown rust (LrAsp5), is intercrossed with a line containing a gene of resistance to powdery mildew (Pm) from the genome of rye Secale cereale, and plants of the generation F1 are self-pollinated to the generation F2. From the generation F2 using molecular PCR marker Pr1/Pr5 the plants are selected containing genes VRN-Asp1 and LrAsp5 in the homozygous state. The plants F2 selected by the PCR marker Pr1/Pr5 with the genes VRN-Asp1 and LrAsp5 are checked by the PCR marker SCM009 to identify the plants with the gene Pm of resistance to powdery mildew.;EFFECT: use of the claimed method enables to simplify the known method and to create the lines of soft spring wheat with an elongated period of earing and with complex resistance to fungal diseases.;2 dwg, 3 tbl
机译:技术领域本发明涉及农业和生物技术。春软小麦的杂交品系与含有两个来自拟南芥的基因的染色体片段:决定穗期延长的基因(VRN-Asp1)和抗褐锈病的基因(LrAsp5)杂交。黑麦Secale谷类的基因组具有抗白粉病(Pm)的基因,而F 1 世代的植物自花授粉至F 2 世代。从使用分子PCR标记Pr1 / Pr5的F 2 世代中,选择含有纯合子基因VRN-Asp1和LrAsp5的植物。通过PCR标记SCM009检查由PCR标记Pr1 / Pr5选择的具有基因VRN-Asp1和LrAsp5的植物F 2 ,以鉴定具有抗白粉病基因Pm的植物。 :使用所要求保护的方法能够简化已知方法,并创建具有更长的穗期和对真菌疾病的复杂抵抗力的软春小麦品系。2dwg,3 tbl

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