HUMAN GROWTH HORMONE DERIVATIVE HIGHLY RESISTANT TO PROTEOLYTIC DEGRADATION, METHOD FOR PREPARING THIS DERIVATIVE, USING IT, METHOD OF TREATING, AND PHARMACEUTICAL COMPOSITION

摘要

FIELD: medicine.;SUBSTANCE: invention refers to molecular biology, medicine, biochemistry and gene engineering. Presented is a human growth hormone derivative containing an additional disulphide bond as compared to hGh defined by SEQ ID No. 1, wherein the derivative contains at least one pair of mutations described by H21C/M170C, D26/V102C, D26/Y103C, F54C/Y143C, F54C/S144C, S55C/Y143C, S57C/Y143C, I58C/Q141C, I58C/Y143C, I58C/S144C, P59C/Q137C, S71C/S132C, L81C/Y143C, Q84C/Y143C, S85C/Y143C, S85C/S144C, F92C/T148C and/or R94C/D107C in SEQ ID No. 1, and possesses the activity of the human growth hormone, as well as a method for preparing this derivative, using it, a method of treating and a pharmaceutical composition.;EFFECT: invention possesses high stability and resistance to proteolytic degradation as a consequence of the introduction of cysteine residues.;18 cl, 3 dwg, 6 tbl, 5 ex