INTEGRATIVE GENETIC CONSTRUCTION pWpr-le

摘要

FIELD: medicine, pharmaceutics.;SUBSTANCE: invention relates to field of biotechnology, in particular to integrative genetic construction pWpr-le. Claimed invention can be used for introduction of gene of human/swine growth hormone releasing factor into chromosome of cell of Bacillus subtilis and related species of genus Bacillus and its further expression in said cells. Integrative genetic construction pWpr-le is characterised by nucleotide sequence, given in Fig. 2.;EFFECT: claimed invention makes it possible to obtain vector system for expression of gene of GHRH_le peptide in bacteria, possessing high stability in obtaining bacterial preparation of probiotic purpose in industrial conditions.;4 dwg, 3 ex