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SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND THEIR USE IN METHOD OF DETECTION AND DIFFERENTIATION OF GENOME OF VACCINE STRAIN B-82 OF MYXOMA VIRUS OF RABBITS FROM FIELD ISOLATES BY METHOD OF POLYMERASE CHAIN REACTION IN REAL TIME
SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND THEIR USE IN METHOD OF DETECTION AND DIFFERENTIATION OF GENOME OF VACCINE STRAIN B-82 OF MYXOMA VIRUS OF RABBITS FROM FIELD ISOLATES BY METHOD OF POLYMERASE CHAIN REACTION IN REAL TIME
FIELD: biotechnology.;SUBSTANCE: method of detection and differentiation of a genome of the vaccine strain B-82 of the myxoma virus of rabbits from the field isolates comprises: extraction of the DNA from the biological material, posing a multiplex polymerase chain reaction using synthesised primers complementary to regions of genes M130R and M151R of the myxoma virus of rabbit, and having the following nucleotide composition: MF 5'TGG-AGC-TTT-TCA-AGC-ATT 3', MR 5'ATA-TCT-CGG-CTC-TAG-GGC-GAG 3', MZ 5' [FAM]-AG-CGT-CGG-ACG-TCT-TCG-TT-[RTQ1] 3', VF 5'AGC-CCT-ATA-AAC-CCG-TAG-ACG-AAC 3', VR 5'CAA-GCT-TTT-TTT-TAT-CCT-CGT-CCG 3', VZ 5' [R6G]-TCG-ACG-GTT-TCG-TCC-GCC-TTC-TTG-[BHQ2] 3', DNA amplification of the virus and evaluation of the reaction. The present inventions may be used in veterinary virology for the detection and differentiation of the vaccine strain B-82 of the myxoma virus of rabbits from the field isolates.;EFFECT: increase in accuracy.;2 cl, 6 tbl, 6 ex
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