METHOD OF DETERMINING SENSITIVITY OF STRAINS Pseudomonas aeruginosa TO ANTIBIOTICS

摘要

FIELD: biotechnology.;SUBSTANCE: method of determining sensitivity of fluorescence spectra comprises cultivation of P. aeruginosa strains on nutrient media, stimulating the synthesis of pioverdine, centrifugation and filtration of cultures for obtaining samples, sample irradiation with spontaneous ultraviolet radiation in the wavelength range of 200-300 nm, obtaining the fluorescence spectra, the resistance marker of the strain to antibiotics is the presence in a band of fluorescence of the peak in the wavelength range of 435-445 nm. The sensitivity marker of the strain to antibiotics is the presence in a band of fluorescence of the peak in the wavelength range of 455-470 nm.;EFFECT: invention enables to simplify the technology of optical methods of the study and to optimise treatment of bacterial infections.;2 dwg