METHOD FOR SPECIFIC DETECTION OF Trichophyton mentagrophytes IN CLINICAL MATERIAL IN VARIOUS CLINICAL FORMS OF DISEASES

摘要

FIELD: medicine.;SUBSTANCE: total native DNA is recovered from skin and hair samples; that is followed by carrying out a polymerase chain reaction and amplification of 5.8S rRNA gene fragment of Trichophyton mentagrophytes with using specific primers. If the electrophoresis procedure enables an amplification product of 182 base pairs in size being detected, the agent is considered to be found.;EFFECT: invention provides the specific detection of Trichophyton mentagrophytes in the clinical material and can be used for diagnosing zooantroponoze trichophytia.;2 ex