DIFFERENTIATION METHOD OF BIOVARS AND GENOVARIATIONS OF Yersinia pestis STRAINS OF MAIN SUBSPECIES BY MEANS OF POLYMERASE CHAIN REACTION

摘要

FIELD: biotechnologies.SUBSTANCE: method provides for separation of DNA of the test strain, series performance of a polymerase chain reaction (PCR) with Med24, glpD, pCKF primers on a DNA target and with 1.ANT/1.ORI, 2.ANT/2.MED primers of the following composition: Med24-S GTATTTTGTGTCACCCC Med24-As AATGAGACACCGCCAGT glpD-F1 GGCTAGCCGCCTCAACAAAAACAT glpD-R1 GGTCATACAAGAACAAGCCGGTGC pCKF-S TCCGTGCTCATTGGTTCG pCKF-As AGACTTGGCGAACGTGGT 1.ANT/1.ORI-S CGTTCTGCTCTCTGTTTGTC 1.ANT/1.ORI-As GTAGAGATGTGTTGCCCG 2.ANT/2.MED-S AAGACCTTCGCCACCAGA 2.ANT/2.MED-As CCAGGATTCGCCGATTCA. Differentiation is performed by comparison of availability and sizes of the formed amplified fragments of the test strain with dimensions of fragments of typical strains of ancient (genovariations 0.ANT, 1.ANT, 2.ANT), mediaeval (genovariations 2.MED, 2.MED0) and eastern (genovariation 1.ORI) biovars.EFFECT: invention allows quick and effective separation of strains of plague originator as to their biovar and intrabiovar belonging.1 tbl, 3 ex