a method of detecting and distinguishing fluids from forensic material

摘要

many sex crimes or other acts of violence for the reconstruction of the facts, the allocation of the available biological traces to the relevant body fluids or tissues. for this purpose, the previously used biochemical parameters, namely proteins. rna from sun, moisture, and microbes are quickly destroyed.the typical biological spurprobe on scene is not fresh, but some years old. in contrast to protein or rna is extremely stable against the mentioned influences dna. dna itself can not be used to distinguish different types of cells, because it is equal in each cell type.with the exception of methylation, methyl (ch3) group a can in the human cell to a cytosine to be committed. in many cases the methyl groups and the controls the activation of a gene. right now, it is very safe, whether on a specific place in the genome such that genaktivitu00e4t driving methyl group is located.methyl groups are covalently bound to the dna and just as stable as this. this makes the methylation at an ideal substrate for the problem of forensic ku00f6rperflu00fcssigkeitsanalyse. this patent pending procedure is the detection of peripheral blood menstrualblut, vaginalflu00fcssigkeit, semen, saliva and skin from forensic material.the procedure is based on the above zellspezifischen methylation of specific genes.