Multiplex pyrosequencing using non-interfering, noise-blocking polynucleotide identification tags

摘要

The present disclosure generally relates to a multiplexing method for analyzing samples comprising polynucleotide amplification for the production of amplified products, wherein one or more target sequences are tagged with a non-interfering, non-terminating, target-specific polynucleotide identification tag, pyrosequencing the amplified products via the sequence of the non-canceling, Target specific polynucleotide identification tag for detecting the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag is correlated with the presence of a specific target sequence.