Multiplex pyrosequencing using non-interfering noise cancelling polynucleotide identification tags
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机译:使用无干扰的消除噪音的多核苷酸鉴定标签进行多重焦磷酸测序
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摘要
The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non-canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.
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