Detection method using recombinant living cells for the detection of xenobiotic substances and arrangement and test kit for performing the detection method

摘要

Known detection methods for foreign substances mark gene sequences of living cells and detect the spatially resolved translocation of stably expressed labeled fusion constructs within the recombinant cells under the influence of foreign substances. The detection method according to the invention uses the dynamic expression behavior of recombinant living cells and detects integrally. The cell-specific defense system is preferably used, whereby gene sequences ai-mi in recombinant cells (19) encode as preferred means in a test kit for carrying out the detection method for transporter proteins Ai with fluorescent marker proteins Mi as fusion constructs Ai-Mi. Under the influence of foreign substances, the cell's own defense system is activated. Transporter proteins Ai are increasingly expressed, which transport the foreign substance out of the living cells (19). Together with the transporter proteins Ai, the marker proteins Mi are increasingly expressed, which is optically detected and allows appropriate conclusions about the foreign substance (03). Preferably, the detection method is implemented with an arrangement (04) of genetically modified aquatic organisms (06) or their living cells (19), which in a water-permeable Habitatbehälter (05) in an aquatic system (01) in the environment of a technical system (02 ) are permanently exposed.