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DETECTION METHOD USING RECOMBINANT LIVING CELLS FOR DETECTING XENOBIOTIC SUBSTANCES AND ARRANGEMENT AND TEST KIT FOR PERFORMING THE DETECTION METHOD
DETECTION METHOD USING RECOMBINANT LIVING CELLS FOR DETECTING XENOBIOTIC SUBSTANCES AND ARRANGEMENT AND TEST KIT FOR PERFORMING THE DETECTION METHOD
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机译:重组活细胞检测异种物质的检测方法及检测试剂盒的排列方法
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摘要
Dynamic expression behavior of recombinant living cells is used and detects translocation in an integral manner, preferably using a cell-inherent defense system, preferably gene sequences, ai-mi, in recombinant cells in a test kit for performing the detection method code for transporter proteins, Ai, with fluorescent marker proteins, Mi, as fusion constructs, Ai-Mi. The cell-inherent defense system is activated under the influence of foreign substance. Transporter proteins, Ai, that transport the foreign substance out of the living cells are expressed in an increased manner. Together with the transporter proteins, Ai, the marker proteins, Mi, are also expressed in an increased manner, which is optically detected and enables corresponding conclusions about the foreign substance. The detection method is preferably implemented using an arrangement of genetically modified aquatic organisms or living cells thereof, which are permanently exposed in a water-permeable habitat tank in an aquatic system in the vicinity of a technical installation.
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