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METHOD OF STABILIZING SUSPENSIONS OF ERYTHROCYTES ENCAPSULATING AN ACTIVE INGREDIENT, SUSPENSIONS OBTAINED
METHOD OF STABILIZING SUSPENSIONS OF ERYTHROCYTES ENCAPSULATING AN ACTIVE INGREDIENT, SUSPENSIONS OBTAINED
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机译:稳定红细胞悬浮液的活性成分,获得悬浮液的方法
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摘要
A method for obtaining a stabilized suspension of red blood cells encapsulating an active ingredient, from resealed RBCs incorporating the active ingredient, the method comprising the incubation of the resealed RBCs in an incubation solution at an osmolality of no less than 280 m Osmol/kg, for a time of 30 minutes or more, the incubation solution being a solution that does not contain an agent which is denaturating for the RBC membrane, the liquid medium is then removed from the incubated suspension and the RBCs obtained are placed in suspension in a solution allowing the injection of the suspension in a patient. The suspensions obtained are particularly characterized by an extracellular haemoglobin level maintained at 0.5 or lower, in particular 0.2 g/dl or lower and/or a hemolysis rate maintained at 2 or less, in particular 1% or less, at 72 h after placing in suspension in a preservation solution and at a temperature of between 2 and 8° C.
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机译:一种从含有活性成分的再密封红细胞中获得稳定化的红细胞稳定悬浮液的方法,该方法包括在渗透溶液中以不小于280 m Osmol / kg的摩尔渗透压浓度孵育再密封的红细胞,在30分钟或更长时间的时间内,孵育溶液是不包含使RBC膜变性的试剂的溶液,然后从孵育的悬浮液中除去液体介质,并将获得的RBC悬浮在溶液中允许将悬浮液注射到患者体内。所获得的悬浮液的特征尤其在于,在放置于水中72小时后,细胞外血红蛋白水平保持在0.5或更低,特别是0.2g / dl或更低,和/或溶血率保持在2或更低,特别是1%或更低。悬浮在保存溶液中,温度在2至8°C之间。
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