METHOD FOR TREATING A SOLUTION IN ORDER TO DESTROY ANY RIBONUCLEIC ACID AFTER AMPLIFICATION

摘要

The present invention provides a method for treating a solution containing among others, target nucleic acid to be amplified in order to destroy any ribonucleic acid that is present in the solution and that could possibly be amplified in another assay. The method is useful to avoid carry-over contamination between experiments. Thus, the present invention provides a method for treating a solution containing at least one target nucleic acid of interest to be amplified comprising the following steps: contacting suitable amplification reagents and at least one target nucleic acid in the presence of i. at least one ribonuclease (RNase), and ii. one ribonuclease inhibitor, the RNase being inhibited by the ribonuclease inhibitor; amplification reagents, RNase, ribonuclease inhibitor and target nucleic acid form the solution; performing a transcription-based amplification of the target; treating said solution in order to inactivate the ribonuclease inhibitor and activate the RNase; degrading any RNA that is present in the solution by the action of said RNase. The present invention is especially useful in methods for diagnostic, preventive and therapeutic applications.