GENOME EDITING OF ROSA LOCUS USING ZINC FINGER NUCLEASE

摘要

PROBLEM TO BE SOLVED: To provide a fusion protein mainly comprised of a nuclease capable of genome-editing of a ROSA gene region known to be ubiquitously expressed.SOLUTION: Provided is a fusion protein in which a nuclease such as a restriction enzyme FokI is fused to a zinc finger designed to have a specific amino acid sequence capable of recognizing the DNA sequence of ROSA gene region. In certain embodiments of the fusion protein, the nuclease comprises a cleavage domain or cleavage half-domain, which is a type IIS restriction endonuclease cleavage domain or a cleavage half-domain.SELECTED DRAWING: Figure 1