Established efficient way of induced pluripotent stem cells

摘要

present invention provides a method for producing iPS cells comprising the following steps: (i) a step of introducing the reprogramming factors into a somatic cell; (ii) Step (i) in cells obtained the step of culture between the following long and 29 days from 11 days; (iii) a process step of sorting the TRA-1-60-positive cells from cells obtained in (ii); (iv). step culturing TRA-1-60 positive cells were sorted in (iii); colonies obtained by (v) step (iv), the step is transferred to another culture vessel; and (vi) culturing the cells obtained in step (v), to obtain iPS cells process. The cells obtained by the step (v), it is preferable that more than 10 passages in culture. The present invention includes inducing differentiation of iPS cells obtained by the above method, the residual ratio of undifferentiated cells is provided the manufacturing method of the reduced differentiated cell populations.FIELD 4