BACTERIAL STRAINS FOR EXPRESSING RECOMBINANT PROTEINS HAVING CHAPERONE-ACTIVITY-RETAINING PROTEASE DEFICIENT DegP AND KNOCKOUT Tsp AND ptr GENES

摘要

PROBLEM TO BE SOLVED: To provide new bacterial strains which provide advantageous means for producing recombinant proteins.;SOLUTION: A recombinant gram-negative bacterial cell comprises one or more of the following mutated protease genes a-c: a. a mutated Tsp gene which encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b. a mutated ptr gene which encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c. a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity. The recombinant gram-negative bacterial cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.;SELECTED DRAWING: Figure 2;COPYRIGHT: (C)2016,JPO&INPIT