METHODS OF MONITORING PATHOLOGICAL CONDITIONS BY SEQUENCE ANALYSIS

摘要

PROBLEM TO BE SOLVED: To provide a method of monitoring the repertoire of T-cell receptor (TCR) and/or B-cell receptor (BCR) of an individual.SOLUTION: In a method, the complementarily determining region 3 (CDR3) from T-cell receptor genes or immunoglobulin genes of T cells and/or B cells is amplified and isolated, and then sequenced by synthesis using reversibly terminated labeled nucleotides; from the determined CDR3 sequence levels, the repertoire of T-cell receptor or B-cell receptor is monitored.SELECTED DRAWING: None