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Isolation and purification of anti-IL-13 antibody using protein A affinity chromatography
Isolation and purification of anti-IL-13 antibody using protein A affinity chromatography
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机译:蛋白A亲和色谱法分离纯化抗IL-13抗体
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摘要
Disclosed herein are methods for the isolation and purification of anti- IL- 13 antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography (e.g., Protein A affinity chromatography), ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
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