Quantitative soft agar colony formation assay using tetrazolium to produce a water soluble formazan

摘要

The present invention aims to provide a rapidly performed it can and measurement accuracy is high quantitative soft agar colony formation assay, specifically, is overlaid with agar containing cells on the agar in the bottom of the container, the cell and overlaid with medium top agar containing a step of culturing the cells, the medium was removed, added and tetrazolium and an electron carrier to produce a water-soluble formazan, a step of culturing cells, color development of a water-soluble formazan The a step of assessing the viability of the cells as an indicator, to a method for evaluating cell survival.