NOVEL DISULFONATED TETRAZOLIUM SALT THAT CAN BE REDUCED TO A WATER-SOLUBLE FORMAZAN AND ITS APPLICATION TO THE ASSAY OF LACTATE DEHYDROGENASE

摘要

A new tetrazolium salt, 4-[3-(1-iodophenyl)-2-(2,4-dinitriphenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate, sodium salt, that produces a highly water-soluble formazan dye upon reduction by reduced nicotinamide adenine dinucleotide (NADH) was synthesized. The reduction of the compound by NADH at a neutral pH is fast owing to its small reduction potential, The applicability of the compound to the assay of lactate dehydrogenase is described in comparison with a prevalent tetrazolium salt. [References: 7]