Primers with modified phosphate and modified bases in the allele-specific PCR

摘要

The present invention is, but at least partially complementary to a plurality of mutant sequence of the target sequence, but have complementary at least one selective nucleotide only one mutated sequence of the target sequence, and includes a nucleotide having a base exocyclic amino group is covalently modified, both modified phosphoric acid utilizing integrated allele-specific oligonucleotide, a method for allele-specific amplification .