[Topic] considering the electric field strength which differs between the sensor structures, amendment the fluorescent tonnage of the measuring object material, offer the fluorescent method of detection which it tries to be able to contrast the measurement result between the sensor structures accurately.Irradiating excited light in the angle of incidence which becomes total reflection conditions from aforementioned dielectric component side, [settlement means] the reaction formation which was formed to the surface of the metal thin film and the aforementioned metal thin film which were formed to the surface of the dielectric component and the aforementioned dielectric component, making use of the sensor structure which the empty at least is formed, to make the electric field on the aforementioned metal thin film reinforce, to make the fluorophor excite which the analyte which is captured to aforementioned reaction formation by the electric field which was reinforced the indicator is done, this being the fluorescent method of detection which at optical detection means it tries to measure the fluorescent tonnage from the fluorophor which was excited, 1stAmendment the fluorescent tonnage which was measured by irradiating excited light under aforementioned 1st measurement conditions the 1st dispersion light intensity and the aforementioned 1st measurement condition which were measured to the occasion where excited light was irradiated under measurement conditions the 2nd dispersion light intensity which was measured to the occasion where under the 2nd measurement conditions for differing excited light was irradiated and, of on the basis.
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