object of the present invention, compared with conventional methods, is to provide a simple and quick method or biomarkers that contribute to discover mastitis early. The above object is cyclophilin A quantity in the mammary gland or milk as an indicator, it is solved by the test method of breast disease. Specifically, the object comprises the following steps (1) and (2), is solved by the inspection method of breast disease. (1) Step (2) to obtain the cyclophilin A content in the milk by detecting cyclophilin A in milk taken from the breast or milk quarters of subjects cyclophilin A in said milk based on the amount, determine the possibility of onset of the onset or mammary gland disease of breast disease in the breast or breast quarters of the subject process
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