Method for manufacturing a large area overlapping in Aspergillus chromosome

摘要

It provides a technique to be duplicated over a wide range of die area any chromosome of bacteria belonging to the genus Aspergillus, the purpose of the present invention, acquisition of the bacteria belonging to the genus Aspergillus with new traits were acquired impossible conventional It is to enable a stable and planned manner, the present invention is disposed on the outside of the 5 'and 3' ends of the region so as to sandwich any area of ​​the chromosome sequence of the transformation marker gene, transformants duplication of chromosome occurs by homologous recombination after the double-strand breaks that occur in the homologous region of the interior of the gene, and, on the chromosome of the bacteria belonging to the genus Aspergillus by culturing the transformant I relates to a method to duplicate any area of ​​the.