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Method for measuring metabolic rate or glucose consumption rate of cells or tissues with high spatiotemporal resolution using glucose nanosensor
Method for measuring metabolic rate or glucose consumption rate of cells or tissues with high spatiotemporal resolution using glucose nanosensor
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机译:利用葡萄糖纳米传感器以高时空分辨率测量细胞或组织的代谢率或葡萄糖消耗率的方法
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摘要
A method to measure the metabolic rate or rate of glucose consumption of cells or tissues with high spatiotemporal resolution using a glucose nanosensor is disclosed. The method generally includes providing single cells or cell populations, cells in suspension or adherent, in a cell culture, a tissue culture, a mixed cell culture, a tissue explant, or in animal tissues in vivo for the measurement of glucose metabolic rate; expressing a glucose sensor in individual cells; calibrating the sensor in controlled conditions; disrupting the steady-state of glucose entering the cell; recording the output from the sensor and calculating the corresponding glucose concentration at different times; and determining the glucose metabolic rate. The method can be applied for the screening of molecules with pharmacological potential, determination of glucose rate of cancerous cells, tissue physiology and biochemistry research.
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