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METHODS AND SYSTEMS FOR VOLUME VARIATION MODELING IN DIGITAL PCR
METHODS AND SYSTEMS FOR VOLUME VARIATION MODELING IN DIGITAL PCR
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机译:数字PCR体积变化建模的方法和系统
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摘要
A method for performing digital polymerase chain reaction (dPCR) is provided. The method includes partitioning a biological sample volume including a plurality of target nucleic acids into a plurality of partitions, where at least one partition includes at least one target nucleic acid. The method further includes determining a model for volume variation of the plurality of partitions and determining a number of partitions including at least one target nucleic acid. The method includes generating a concentration of target nucleic acids in the biological sample based on the model for volume variation and the fraction of partitions including at least one target nucleic acid.
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