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METHOD AND SYSTEM FOR STOKES INTERFERENCE STIMULATED FLUORESCENT SCATTERING FOR IN-VIVO IMAGING
METHOD AND SYSTEM FOR STOKES INTERFERENCE STIMULATED FLUORESCENT SCATTERING FOR IN-VIVO IMAGING
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机译:斯托克斯干扰激发荧光成像的方法和系统
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摘要
A microscopy system includes a first laser emitting a first laser pulse, the first laser pulse being a pump beam; a second laser emitting a second laser pulse, the second laser pulse being spectrally isolated for generating a probe beam and a donut beam; an optical device for combining the pump beam, the probe beam and the donut beam into a combined laser pulse, the probe beam and donut beam having a phase difference that causes a reduction of a focal volume of the combined laser pulse; a galvanometer scanning system for delivering the combined laser pulse to a focal spot in a focal plane, wherein the reduction of the focal volume of the combined laser pulse initiates a stimulated emission of a targeted molecule, the stimulated emission having dipole-like backscatter; and a sensor for enabling imaging of the dipole-like backscatter.
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