Integrated cultivation and measurement device for label-free detection and classification of cellular alterations, in particular for generation and characterisation of cell-spheroids, components and uses thereof

摘要

In a method for label-free detection and classification of cellular alterations, cells, cell-spheroids or tissue samples are introduced in culture reservoirs of a cultivation chamber plate. The cells, cell-spheroids or tissue sample are positioned in the microcavities of the cultivation chamber plate by movement of a rotary shaker that is provided with an amplifier board and mounting device on which the cultivation chamber plate is mounted. The impedance of the cells, cell-spheroids or tissue samples between two microelectrodes and/or electrogenic activity of the cells, cell-spheroids or tissue sample on every microelectrode and their changes during the cultivation and between different culture reservoirs are determined.