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REAL-TIME, LABEL-FREE DETECTION OF MACROMOLECULES IN DROPLETS BASED ON ELECTRICAL MEASUREMENTS
REAL-TIME, LABEL-FREE DETECTION OF MACROMOLECULES IN DROPLETS BASED ON ELECTRICAL MEASUREMENTS
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机译:基于电气测量的果蔬中宏分子的实时,无标记检测
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摘要
A method for detecting presence of a macromolecule of interest in a test droplet. A set of detection electrodes are provided in contact with a fluidic channel. The test droplet is provided in vicinity of the detection electrodes through the fluidic channel. An alternate current (AC) power at a first frequency is applied across the set of detection electrodes. A first measurement value that reflects electrical characteristics (e.g., impedance) of the test droplet at the first frequency is obtained. This value is compared with a corresponding reference value, wherein the corresponding reference value is obtained by measuring a reference droplet containing the macromolecule of interest or free of the macromolecule at the first frequency. The presence of the macromolecule in the test droplet is thus determined based on the comparison.
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