METHOD FOR CALIBRATING A LASER DEFLECTION APPARATUS OF A LASER MICRODISSECTION SYSTEM AND LASER MICRODISSECTION SYSTEM

摘要

A method for calibrating a laser deflection device in a reflected light device of a microscope of a laser inictodissection system having a digital image capturing unit comprising an image evaluation module includes generating a laser beam; guiding the laser beam through a microscope objective; directing the laser beam to a position defined by actuation sinals; placing a calibration object in the object plane of the microscope objective; actuating the laser deflection device using first actuation signais and first calibration values, making at least one calibration mark on the calibration object; capturing an image ofthe calibration object by the digital image capturing unit; determining actual position values for the at least one calibration mark; and determining second calibration values based on a relationship between the default position values and the actual position values.