首页> 外国专利> METHOD, KIT AND PREMIX FOR QUANTITATIVE AND DISCRIMINATIVE DETERMINATION OF NUCLEIC ACIDS, QDPCR; IN VITRO METHOD FOR THE QUANTITATIVE AND DISCRIMINATIVE DIAGNOSIS OF BIOLOGICAL ENTITIES

METHOD, KIT AND PREMIX FOR QUANTITATIVE AND DISCRIMINATIVE DETERMINATION OF NUCLEIC ACIDS, QDPCR; IN VITRO METHOD FOR THE QUANTITATIVE AND DISCRIMINATIVE DIAGNOSIS OF BIOLOGICAL ENTITIES

机译：定量和鉴别测定核酸，QDPCR的方法，试剂盒和预混合试剂盒;定量和鉴别性诊断生物实体的体外方法

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摘要

The present invention provides a process and a set of compounds of a kit for quantitative, specific and discriminative determination of nucleic acids (qdPCR). The products and processes of the invention correspond to a development which enables, at the same time and in a single closed system, to obtain a quantitative diagnosis of a gene locus, associated to a determinative and discriminative method between alleles or gene loci or even between orthologous genes of different biological entities present in samples/assays.

机译：本发明提供了用于核酸的定量，特异性和区分性测定（qdPCR）的试剂盒的方法和一组化合物。本发明的产品和方法对应于一种发展，其使得能够同时在单个封闭系统中获得与等位基因或基因座之间或什至之间的确定性和区分性方法有关的基因座的定量诊断。样品/测定中存在的不同生物实体的直系同源基因。

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公开/公告号US2016273031A1

专利类型
公开/公告日2016-09-22

原文格式PDF
申请/专利权人 SPINOMICS LTD. - EPP;NEOPROSPECTA PESQUISA E CONSULTORIA S.A.;
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申请/专利号US201415037123
发明设计人 ROGÉRIO MARGIS;LUIZ FELIPE VALTER DE OLIVEIRA;
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申请日2014-11-14
分类号C12Q1/68;
国家 US
入库时间 2022-08-21 14:38:02

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Poster session 2Morphogenetic mechanisms290MiR-133 regulates retinoic acid pathway during early cardiac chamber specification291Bmp2 regulates atrial differentiation through miR-130 during early heart looping formationDevelopmental genetics294Association of deletion allele of insertion/deletion polymorphism in alpha 2B adrenoceptor gene and hypertension with or without type 2 diabetes mellitus295Association of G1359A polymorphism of the endocannabinoid type 1 receptor (CNR1) with coronary artery disease (CAD) with type 2 diabetes mellitusCell growth, differentiation and stem cells - Vascular298Gamma-secretase inhibitor prevents proliferation and migration of ductus arteriosus smooth muscle cells: a role of Notch signaling in postnatal closure of ductus arteriosus299Mesenchymal stromal-like cells (MLCs) derived from induced pluripotent stem (iPS) cells: a promising therapeutic option to promote neovascularization300Sonic Hedgehog promotes mesenchymal stem cell differentiation to vascular smooth muscle cells in cardiovacsular disease301Proinflammatory cytokine secretion and epigenetic modification in endothelial cells treated LPS-GinfivalisCell death and apoptosis - Vascular304Mitophagy acts as a safeguard mechanism against human vascular smooth muscle cell apoptosis induced by atherogenic lipidsTranscriptional control and RNA species - Vascular307MicroRNA-34a role in vascular calcification308Local delivery of a miR-146a inhibitor utilizing a clinically applicable approach attenuates neointima formation after vascular injury309Long noncoding RNA landscape of hypoxic endothelial cells310Specific circulating microRNAs levels associate with hypertension, hyperglycemia and dysfunctional HDL in acute coronary syndrome patientsCytokines and cellular inflammation - Vascular313Phosphodiesterase5A up-regulation in vascular endothelium under pro-inflammatory conditions: a newly disclosed anti-inflammatory activity for the omega-3polyunsaturated aatty acid docosahexaenoic acid314Cardiovascular risk modifying with extra-low dose anticytokine drugs in rhematoid arthritis315Conversion of human M-CSF macrophages into foam cells reduces their proinflammatory responses to classical M1-polarizing activation316Lymphocytic myocarditis coincides with increased plaque inflammation and plaque hemorrhage in coronary arteries, facilitating myocardial infarction317Serum osteoprotegerin level predictsdeclined numerous of circulating endothelial- derived and mononuclear-derived progenitor cells in patients with metabolic syndromeGrowth factors and neurohormones - Vascular320Effect of gastrin-releasing peptide (GRP) on vascular inflammationSignal transduction - Heart323A new synthetic peptide regulates hypertrophy in vitro through means of the inhibition of nfkb324Inducible fibroblast-specific knockout of p38 alpha map kinase is cardioprotective in a mouse model of isoproterenol-induced cardiac hypertrophy325Regulation of beta-adrenoceptor-evoked inotropic responses by inhibitory G protein, adenylyl cyclase isoforms 5 and 6 and phosphodiesterases326Binding to RGS3 and stimulation of M2 muscarinic acetylcholine receptors modulates the substrate specificity of p190RhoGAP in cardiac myocytes327Cardiac regulation of post-translational modifications, parylation and deacetylation in LMNA dilated cardiomyopathy mouse model328Beta-adrenergic regulation of the b56delta/pp2a holoenzyme in cardiac myocytes through b56delta phosphorylation at serine 573Nitric oxide and reactive oxygen species - Vascular331Oxidative stress-induced miR-200c disrupts the regulatory loop among SIRT1, FOXO1 and eNOS332Antioxidant therapy prevents oxidative stress-induced endothelial dysfunction and Enhances Wound Healing333Morphological and biochemical characterization of red blood cell in coronary artery diseaseCytoskeleton and mechanotransduction - 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