Refolding Proteins Using a Chemically Controlled Redox State

摘要

A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L, or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox butler strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.