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METHOD FOR SPECIES IDENTIFICATION BY USING MOLECULAR WEIGHTS OF NUCLEIC ACID CLEAVAGE FRAGMENTS
METHOD FOR SPECIES IDENTIFICATION BY USING MOLECULAR WEIGHTS OF NUCLEIC ACID CLEAVAGE FRAGMENTS
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机译:核酸裂解片段分子量的物种鉴定方法
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摘要
A method for species identification by using molecular weights of nucleic acid cleavage fragments, comprising steps of: performing a polymerase chain reaction and a nucleic acid cleavage reaction to cleave the nucleic acid sequence of the to-be-identified species into multiple nucleic acid cleavage fragments having different molecular weights; measuring the molecular weights of the nucleic acid cleavage fragments by using a mass spectrometer; comparing the molecular weight of each nucleic acid cleavage fragments of the to-be-identified species with molecular weights of nucleic acid cleavage fragments of a known species in a database; determining a number N of the identical nucleic acid cleavage fragments between the two species; and calculating a ratio N/M of the number N to the total number M of the nucleic acid cleavage fragments of the known species, wherein the ratio N/M represents similarity between the to-be-identified species and the known species.
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