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CHEMICALLY DEFINED ALBUMIN-FREE CONDITIONS FOR CARDIOMYOCYTE DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS
CHEMICALLY DEFINED ALBUMIN-FREE CONDITIONS FOR CARDIOMYOCYTE DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS
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机译:化学定义的无蛋白条件用于人类多能干细胞分化的心肌细胞
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摘要
Methods for generating high-yield, high-purity cardiomyocyte progenitors or cardiomyocytes from pluripotent cells are described. Wnt/β-catenin signaling is first activated in pluripotent cells, by, for example, inhibiting Gsk-3 to obtain a first population of cells. Wnt/β-catenin signaling is then inhibited in the first cell population to induce cardiogenesis. One or more of these steps is performed under defined, albumin-free culture conditions.
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