Rapid thermal cycling for PCR reactions using enclosed reaction vessels and linear motion

摘要

A system for fast, accurate and inexpensive thermal cycling is disclosed including a set of thermally conductive plates that are maintained in a fixed spatial relationship to each other, and separated from each other by a thermal insulating space. A hole having a size approximately equal to a size of a desired test sample is formed through the set of thermally conductive plates. The test sample is placed in the hole and moved back and forth between the different temperature plates, to the desired temperature locations for desired time periods, in a pattern that is determined by the user, and repeated as many times as needed for the specific process.