Methods are disclosed for preparing novel biodegradable nanoparticles based on complexation of poly-gamma-glutamic acid (γ-PGA) or its cross-linked derivatives with bivalent lead ions. The final products are stable in aqueous media at low pH, neutral and mild alkaline conditions. The size of the complexes depends on the pH, concentrations and the ratios of γ-PGA and lead ions;The γ-PGA nanoparticles made from biodegradable biopolymers with high flocculating and heavy metal binding activity of the present invention may be used for various water treatment applications in aqueous media.
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