首页> 外国专利> Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)

Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)

机译：通过表达从 Euonymus alatus （燃烧的灌木丛）中分离的乙酰基转移酶基因生产乙酰基二酰甘油（ac-TAG）的方法

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摘要

The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories. 展开▼

机译：本发明涉及新的二酰基甘油酰基转移酶基因和蛋白质，及其使用方法。特别地，本发明描述了编码具有二酰基甘油乙酰基转移酶活性的蛋白质的基因，特别是用于将乙酰基转移至二酰基甘油底物以形成乙酰基-三酰基甘油（ac-TAGS），例如3-乙酰基-1,2-二酰基-sn -甘油。本发明包括转移酶的天然和重组野生型形式，以及突变体和变体形式。本发明还涉及使用新的二酰基甘油酰基转移酶基因和蛋白质，包括它们在转基因生物中以商业上可行的水平表达，以增加植物油中3-乙酰基-1,2-二酰基-sn-甘油的产量并改变植物油的方法。微生物（例如酵母）通过增加ac-TAG产量产生的油的组成。另外，通过本发明的方法生产的包含基因和蛋白质的油被考虑用作生物柴油燃料，用于聚合物生产和具有降低的卡路里的天然生产的食用油。展开▼

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公开/公告号US9328335B2

专利类型

公开/公告日2016-05-03

原文格式PDF

申请/专利权人 TIMOTHY DURRETT;JOHN OHLROGGE;MICHAEL POLLARD;
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申请/专利号US201013519660

发明设计人 JOHN OHLROGGE;TIMOTHY DURRETT;MICHAEL POLLARD;
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申请日2010-12-29

分类号C12N9/10;C12N15/82;C12P7/64;

国家 US

入库时间 2022-08-21 14:29:41

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3. METHOD TO PRODUCE ACETYLDIACYLGLYCEROLS (AC-TAGS) BY EXPRESSION OF AN ACETYLTRANSFERASE GENE ISOLATED FROM EUONYMUS ALATUS (BURNING BUSH) [P] . 美国专利： US2013116462A1 . 2013-05-09

机译：通过表达白僵菌（烧伤）分离的乙酰转移酶基因生产乙二酰甘油（AC-tags）的方法

4. A METHOD TO PRODUCE ACETYLDIACYLGLYCEROLS (AC-TAGS) BY EXPRESSION OFAN ACETYLTRANSFERASE GENE ISOLATED FROM EUONYMUS ALATUS (BURNING BUSH) [P] . 世界知识产权组织专利： WO2011082253A3 . 2011-09-29

机译：一种表达白僵菌（烧伤）分离的乙酰转移酶基因生产乙酰二环甘油（AC-tags）的方法

5. A METHOD TO PRODUCE ACETYLDIACYLGLYCEROLS (AC-TAGS) BY EXPRESSION OFAN ACETYLTRANSFERASE GENE ISOLATED FROM EUONYMUS ALATUS (BURNING BUSH) [P] . 世界知识产权组织专利： WO2011082253A2 . 2011-07-07

机译：一种表达白僵菌（烧伤）分离的乙酰转移酶基因生产乙酰二环甘油（AC-tags）的方法

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